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106223

106223-2.5L



Solvent Mix Pyridine in ACN (V / V = 60 : 40) NC-0612, GL45 thread emp Biotech GmbH


CAS Number: 110-86-1
   
Molecular Formula: C5H5N
   
Molecular Weight: 79.10
   
MDL Number:


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106223-2.5L
Regular Price: $133.00
Your Discounted Price: $0.00
In Stock USA



 
Pictograms:
Signal Word:Danger
Hazard Statements:H225, H315, H319, H335
Precautionary Statements:P210, P280, P305 + P351 + P338
UN#:UN1993
Packing Group:III
Hazard Class:3
Flash Point:
SDS: See MSDS
 
Limited Quantities:5.0 L (1.3 gallons) (liquid)
Excepted Quantities:Max Inner Pack (30g or 30ml) and Max Outer Pack (1Kg or 1L)

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For each synthesis cycle, up to 1 to 2 % of free 5'-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent "Capping" step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences. For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.

Product of emp Biotech GmbH
Size bottle: 2.5 L GL45 thread, emp Biotech part no. NC-0612-N002.5-001

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